The Dengue virus (DENV) (Flaviviridae family, Flavivirus genus), which is transmitted by Aedes aegypti and Aedes albopictus, is the most important arthropod born virus worldwide, with more than 390 millions of human infections occurring every year. Infection with any of the four DENV serotypes (DENV-1, -2, -3 and -4) can be asymptomatic or can lead to a wide spectrum of disease, ranging from a self-limited flu-like illness to a more severe form, characterized by severe plasma leakage and hypovolemic shock that in some cases could be fatal. Epidemiological studies have shown that a secondary infection with a different virus serotype is a risk factor for the development of the more severe form of the disease. Therefore, epidemiological surveillance of DENV serotypes is of great interest for prevention and control programs. In this study, we have showed the specific detection of the four DENV serotypes using a DNA microarray platform, which was designed to detect all known viruses transmitted by arthropods and small mammals. All the four DENV serotypes, obtained either from the supernatant of infected C6/36 cell cultures or the serum of infected patients, were specifically detected. The DNA microarray was also able to specifically identify the DENV serotypes in a mixture, which can be observed in patients during epidemic outbreaks in highly endemic regions. In addition, the DNA microarray showed sensitivity similar to that found for a real-time polymerase chain reaction preceded by a reverse transcription (RT-PCR), suggesting it can be use in routine diagnosis of the disease. However, because of the high cost of the test, it is recommended the use this platform only in the identification of the virus serotypes circulating during epidemic periods and in the surveillance of other viruses transmitted by arthropods and small mammals.